KMID : 1059520100540050573
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Journal of the Korean Chemical Society 2010 Volume.54 No. 5 p.573 ~ p.578
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Study of Interaction of Native DNA with Iron(III)-(2,4-Dihydroxysalophen)chloride
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Azani, Mohammad-Reza
Hassanpour, Azin Bordbar, Abdol-Khalegh
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Abstract
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In this study, iron(III)-2,4-dihydroxysalophen chloride (Fe(2,4-DHSalophen)Cl), has been synthesized by combination of 2,4-dihydroxysalophen (2,4-DHSalophen) with FeCl©ü in a solvent system. This complex combination was characterized using UV-vis and IR spectroscopies. Subsequently, the interaction between native calf thymus deoxyribonucleic acid (ct-DNA) and Fe(2,4-DHSalophen)Cl, was investigated in 10 mM Tris/HCl buffer solution, pH 7.2, using UV-visible absorption and fluorescence spectroscopies, thermal denaturation technique and viscosity measurements. From spectrophotometric titration experiments, the binding constant of Fe(2,4-DHSalophen)Cl with ct-DNA was found to be (1.6¡¾0.2)¡¿10©ø M-©ö. The fluorescence study represents the quenching effect of Fe(2,4-DHSalophen)Cl on bound ethidium bromide to DNA. The quenching process obeys linear Stern-Volmer equation in extended range of Fe(2,4-DHSalophen)Cl concentration. Thermal denaturation experiments represent the increasing melting temperature of DNA (about 4.3¡É) due to binding of Fe(2,4-DHSalophen)Cl. These results are consistent with a binding mode dominated by interactions with the groove of ct-DNA.
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KEYWORD
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DNA, Schiff base, Interaction, Fluorescence, UV-vis spectro
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